Journal: Animals : an Open Access Journal from MDPI

Article Title: Integrated Histological and Transcriptomic Analyses Reveal Distinct Structural and Molecular Features of the Calipash and Muscle in the Chinese Soft-Shelled Turtle ( Pelodiscus sinensis )

doi: 10.3390/ani16182960

Figure Lengend Snippet: All the primers listed in this study.

Article Snippet: After blocking with 10% goat serum (Boster Biological Technology Co., Ltd., Wuhan, China) for 30 min, the sections were incubated with a rabbit anti-COL1A1 primary antibody (ABclonal Biotechnology Co., Ltd., Wuhan, China; 1:200 dilution) overnight at 4 °C.

Techniques: Sequencing

Journal: Animals : an Open Access Journal from MDPI

Article Title: Integrated Histological and Transcriptomic Analyses Reveal Distinct Structural and Molecular Features of the Calipash and Muscle in the Chinese Soft-Shelled Turtle ( Pelodiscus sinensis )

doi: 10.3390/ani16182960

Figure Lengend Snippet: Quantitative analysis of collagen abundance in the calipash and muscle. ( A , B ) Representative Masson’s trichrome-stained sections utilized for collagen quantification in calipash ( A ) and muscle ( B ). ( A1 – A3 ) The first/second/third microscopic field in calipash; ( B1 – B3 ) The first/second/third microscopic field in muscle. The microscopic images are representative fields from the same individual and were used for visualization only. ( C ) Quantitative comparison of the collagen area fraction between the calipash and muscle tissues. Data are presented as mean ± SD ( n = 3) calculated from three independent microscopic fields. * p < 0.05 indicates significant differences.

Article Snippet: After blocking with 10% goat serum (Boster Biological Technology Co., Ltd., Wuhan, China) for 30 min, the sections were incubated with a rabbit anti-COL1A1 primary antibody (ABclonal Biotechnology Co., Ltd., Wuhan, China; 1:200 dilution) overnight at 4 °C.

Techniques: Analysis, Staining, Comparison

Journal: Animals : an Open Access Journal from MDPI

Article Title: Integrated Histological and Transcriptomic Analyses Reveal Distinct Structural and Molecular Features of the Calipash and Muscle in the Chinese Soft-Shelled Turtle ( Pelodiscus sinensis )

doi: 10.3390/ani16182960

Figure Lengend Snippet: Comparative Picro Sirius Red staining of collagen subtype composition and spatial organization. ( A1 – A4 ) Representative brightfield microscopy images of the calipash acquired at increasing magnification. Scale bars: 200 μm ( A1 ), 100 μm ( A2 ), 50 μm ( A3 ) and 20 μm ( A4 ). ( B1 – B4 ) Representative polarized light microscopy images of the calipash acquired at increasing magnification. Scale bars: 200 μm ( B1 ), 100 μm ( B2 ), 50 μm ( B3 ) and 20 μm ( B4 ). ( C1 – C4 ) Representative brightfield microscopy images of muscle acquired at increasing magnification. Scale bars: 200 μm ( C1 ), 100 μm ( C2 ), 50 μm ( C3 ) and 20 μm ( C4 ). ( D1 – D4 ) Representative polarized light microscopy images of muscle acquired at increasing magnification. Scale bars: 200 μm ( D1 ), 100 μm ( D2 ), 50 μm ( D3 ) and 20 μm ( D4 ). Yellow-orange birefringence indicates densely packed type I collagen, while green birefringence is indicative of type III collagen-rich regions. Scale bars are as indicated in the respective images.

Article Snippet: After blocking with 10% goat serum (Boster Biological Technology Co., Ltd., Wuhan, China) for 30 min, the sections were incubated with a rabbit anti-COL1A1 primary antibody (ABclonal Biotechnology Co., Ltd., Wuhan, China; 1:200 dilution) overnight at 4 °C.

Techniques: Staining, Brightfield, Microscopy, Light Microscopy

Journal: Animals : an Open Access Journal from MDPI

Article Title: Integrated Histological and Transcriptomic Analyses Reveal Distinct Structural and Molecular Features of the Calipash and Muscle in the Chinese Soft-Shelled Turtle ( Pelodiscus sinensis )

doi: 10.3390/ani16182960

Figure Lengend Snippet: Comparative immunofluorescence localization of COL1A1 in calipash ( A1 – A3 ) and muscle ( B1 – B3 ) tissues. ( A1 ) Representative immunofluorescence image showing the spatial distribution of COL1A1 (green) in calipash at low magnification; scale bar, 200 μm. ( A2 ) Higher-magnification image of a representative field of view in calipash; scale bar, 50 μm. ( A3 ) Higher-magnification image of a second, independent field of view in calipash; scale bar, 50 μm. ( B1 ) Spatial distribution of COL1A1 (red) in muscle tissue at low magnification; scale bar, 200 μm. ( B2 ) Higher-magnification image of a representative field of view in muscle; scale bar, 50 μm. ( B3 ) Higher-magnification image of a second, independent field of view in muscle; scale bar, 50 μm. Nuclei were counterstained with DAPI (blue). Scale bars are as indicated in the respective images.

Article Snippet: After blocking with 10% goat serum (Boster Biological Technology Co., Ltd., Wuhan, China) for 30 min, the sections were incubated with a rabbit anti-COL1A1 primary antibody (ABclonal Biotechnology Co., Ltd., Wuhan, China; 1:200 dilution) overnight at 4 °C.

Techniques: Immunofluorescence, Tissue

Journal: Animals : an Open Access Journal from MDPI

Article Title: Integrated Histological and Transcriptomic Analyses Reveal Distinct Structural and Molecular Features of the Calipash and Muscle in the Chinese Soft-Shelled Turtle ( Pelodiscus sinensis )

doi: 10.3390/ani16182960

Figure Lengend Snippet: Collagen gene expression profiling, transcriptional validation, and protein-level confirmation in calipash and muscle of P. sinensis . ( A ) Volcano plot of collagen-related DEGs. Each point represents a gene, with significantly upregulated (green) and downregulated (orange) genes in calipash defined by |log 2 fold change| ≥ 1 and FDR < 0.05. The x -axis represents log 2 fold change, and the y -axis represents log 10 adjusted p value. Collagen-related genes are highlighted, including col1a1 , col1a2 , col3a1 , col6a1 , col6a2 , col4a6 , col4a5 , col5a3 , col7a1 , col12a1 , col16a1 , and col17a1 . ( B ) Targeted qPCR validation of col1a1 and col1a2 expression in calipash and muscle. Data are presented as mean ± SD ( n = 3). Asterisks indicate significant differences between calipash and muscle ( p < 0.05). ( C ) Western blot analysis of COL1A1 protein in calipash and muscle. β-actin was used as the loading control.

Article Snippet: After blocking with 10% goat serum (Boster Biological Technology Co., Ltd., Wuhan, China) for 30 min, the sections were incubated with a rabbit anti-COL1A1 primary antibody (ABclonal Biotechnology Co., Ltd., Wuhan, China; 1:200 dilution) overnight at 4 °C.

Techniques: Gene Expression, Profiling, Biomarker Discovery, Expressing, Western Blot, Analysis, Control

Journal: Animals : an Open Access Journal from MDPI

Article Title: Integrated Histological and Transcriptomic Analyses Reveal Distinct Structural and Molecular Features of the Calipash and Muscle in the Chinese Soft-Shelled Turtle ( Pelodiscus sinensis )

doi: 10.3390/ani16182960

Figure Lengend Snippet: DEGs related to collagen in the muscle and calipash of P. sinensis .

Article Snippet: After blocking with 10% goat serum (Boster Biological Technology Co., Ltd., Wuhan, China) for 30 min, the sections were incubated with a rabbit anti-COL1A1 primary antibody (ABclonal Biotechnology Co., Ltd., Wuhan, China; 1:200 dilution) overnight at 4 °C.

Techniques:

Journal: Advanced Science

Article Title: Heat‐Suppressing Projection Two‐Photon Lithography Enables High‐Throughput Sub‐Micrometer Manufacturing of Biopolymer Hydrogels for Tissue Engineering

doi: 10.1002/advs.76938

Figure Lengend Snippet: Topographically engineered HMPs for aligned tissue fabrication. (A) Schematic illustration of pattern‐guided cell orientation. (B) Representative confocal images of cortical neurons cultured on HMPs with surface grooves of varying widths. Scale bars: 20 µm. (C) Quantification of the 90% cell orientation distribution range for cortical neurons on HMPs with different groove widths ( n = 28–30). (D) Schematic illustration of printed tendon‐like microtissue and confocal images of used HMPs. Scale bar: 50 µm. (E) Representative maximum intensity projection confocal images of NIH 3T3 fibroblasts cultured for 3 weeks on scaffolds assembled from HMSs or grooved HMPs using identical extrusion printing parameters (yellow: nucleus; magenta: F‐actin; cyan: Col1a1). Scale bars: 100 µm. (F) Representative orientation maps of nuclear and F‐actin alignment in HMS‐ versus HMP‐based scaffolds after 3 weeks of culture, with the horizontal direction defined as 0°. Scale bars: 100 µm. (G) Angular distribution of nucleus and F‐actin orientation for both scaffold types. (H) Standard deviation of orientation angles for nucleus and F‐actin, indicating alignment consistency ( n = 6). (I) Quantification of Col1a1 fluorescence intensity from single‐slice confocal cross‐sections of the engineered microtissues ( n = 6). (J) Tensile modulus and fracture strength measured from uniaxial tensile tests of HMS‐ and HMP‐based scaffolds cultured with NIH 3T3 fibroblasts for 3 weeks ( n = 3). ** = p < 0.01, **** = p < 0.0001; one‐way ANOVA test for (C) and t‐test for (H–J).

Article Snippet: Col1a1 rabbit pAb (Abclonal A1352, 1:200) was used for collagen quantification.

Techniques: Cell Culture, Standard Deviation, Fluorescence